Nanocomposition of hydroxylapatite from cortical bone tissue
A.A. Bibko, D.V. Lychagin, O.V. Bukharova, E.A. Kostrub, M.O. Khrushcheva
| UDK 549.01:549.02: 549.752:553.086 | https://doi.org/10.35597/2313-545X-2024-10-3-2 | Read PDF (RUS) Views: 1 |
Hydroxylapatite is one of the main components of bone tissue. In combination with collagen, it provides unique strength properties of the bone. The nanostructure of bone tissue (its composition) remains a matter of debate. In this work, we studied the cortical bone tissue of rats using transmission electron microscopy (TEM) and X-ray diffraction. According to the results of the Scherrer method, the size of hydroxylapatite crystallites is 8.8 ? 4.0 nm. The TEM showed the presence of crystalline hydroxyapatite areas in the interfibrillary space of collagen with the sizes ranging from 10 ? 5 to 50 ? 10 nm. These areas are the crystalline aggregates with a subblock structure, which is expressed in different orientations of axis c. No amorphous substance in bone tissue was identified using electron nanodiffraction.
Keywords: hydroxylapatite, bone tissue, biomineral, nanostructure, TEM, Scherrer method.
Funding. This work was supported by state contract no. 0721-2020-0041 of the Ministry of Science and Higher Education of the Russian Federation.
Acknowledgements. Samples for TEM and imaging were prepared using equipment of the Shared Research Center “Nanotech” of the Institute of Strength Physics and Materials Science, Siberian Division, Russian Academy of Sciences (Tomsk, Russia). X-ray diffraction studies were carried out using the equipment of the Tomsk Regional Shared Research Center of the National Research Tomsk State University (Tomsk, Russia). We thank the reviewer for the constructive comments for the improvement of the manuscript.
Conflict of interest. The authors declare that they have no conflicts of interest.
Author contribution. A.A. Bibko, O.V. Bukharova – conceptualization, investigation; E.A. Kostrub, A.A. Bibko – preparation of biological samples; A.A. Bibko, D.V. Lychagin, M.O. Khrushcheva – analytical and experimental work; A.A. Bibko, O.V. Bukharova, D.V. Lychagin – writing – original draft; A.A. Bibko, O.V. Bukharova– writing – review & editing. All authors approved the final version of the article before publication. All the authors approved the final version of the manuscript prior to publication.
For citation: Bibko A.A., Lychagin D.V., Bukharova O.V., Kostrub E.A., Khrushcheva M.O. Nanocomposition of hydroxylapatite from cortical bone tissue. Mineralogy, 10(3), 20–31. DOI: 10.35597/2313-545X-2024-10-3-2
Received 19.04.2024, revised 10.06.2024, accepted 10.07.2024
A.A. Bibko, National Research Tomsk State University, Tomsk, Russia; bibko.geology@gmail.com
D.V. Lychagin, National Research Tomsk State University, Tomsk, Russia;
O.V. Bukharova, National Research Tomsk State University, Tomsk, Russia;
E.A. Kostrub, Siberian State Medical University, Tomsk, Russia;
M.O. Khrushcheva, National Research Tomsk State University, Tomsk, Russia
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